In yet another historic day for the Icelandic Football team, Argentina was held to a 1-1 draw by the tournament debutants at the Spartak stadium tonight.
Messi had a chance to get the victor for Sampaoli's men ten minutes before time with a curling strike from just outside the box but guided his effort slightly wide.
But the Barcelona ace's lackluster record on penalties continued, as he stepped up and fired a weak shot at the flawless height and velocity to gift Iceland keeper Hannes Halldorsson an easy save.
The lead lasted just four minutes though as sloppy defending allowed Iceland to work the ball to Alfred Finnbogason for a simple tap in.
Jorge Sampaoli's men however took the lead 10 minutes later through Sergio Aguero.
Halldorsson, a part-time film director, had his own script in mind and the Randers FC keeper flung himself to his right and made his mark on World Cup history.
Messi stepped up but Iceland goalkeeper Hannes Halldorsson made the save to deny the Argentinian icon his first goal of the tournament. "The game on Saturday - the first one - is always the most important".
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Sergio Ramos' shithousery against the winger took the Liverpool forward out of the Champions League final after just half an hour. Jose Gimenez came to Uruguay's rescue by scoring the lone goal in a 1-0 victory over Egypt on Friday in Group A of the World Cup.
The South Americans are outright favourites to dominate the group along with probably Croatia, notwithstanding the dangers of Nigeria.
Iceland will be looking to neutralize the attack of Lionel Messi, the captain and goal-scorer for Argentina; he has 64 goals for the national team since joining it 14 years ago. In that moment, going to the screen, I saw him standing there, the body language showed he didn't know, and then he had to make a decision between France or Australia. They wrote one of the most attractive stories two years ago and they have full right to hope for the best this summer in Russian Federation with the same group of players on disposal.
Argentina coach Jorge Sampaoli dismissed his star player's penalty set-back as a "statistic" and praised his attitude in a hard game.
"I looked at a lot of penalties from Messi and I also looked at how I've been behaving during the last couple of penalties".
Iceland caught the world's imagination in the last global competition they were a part of; 2016 Euros.
A star-studded Argentina could not beat Iceland.
They said what? Australia coach Bert van Marwijk: "I hope that maybe one time there'll be a referee that's very honest".
Argentina face Croatia next on Thursday at the Nizhny Novgorod Stadium (19:00 BST).